Micro plastic: an imperceptible threat on Agua Dulce Beach, Chorrillos District

The use of plastics in the world is increasing, but their proper final arrangement is limited. Peru made regulatory efforts to reduce its use;however, they are isolated activities that have not represented a significant change in their management. This study allows to identify the presence of microplastic on one of the busiest beaches of the city of Lima Metropolitana, during the emergency period caused by the SARS-COV-2 pandemic. To achieve the objectives of the research, it has been sampled in high tide and supra-coastal areas, applying the methodology proposed in the Ministry of the Environment's Soil Sampling Guide. This study shows the presence of microplastics, with an abundance of 43 pieces per m2, and found that the predominance of the type of microplastic found is of secondary origin (polystyrene and polypropylene);however, 20.9% correspond to primary microplastics (pellets) whose source could not be determined. Finally, it is indicated that despite restrictions on the state of emergency the number of microplastics per m2 in Agua Dulce Beach between 2019 (40 pieces) and 2020 (43 pieces) increased by 7.5%.

Silent superbug killers in a river near you how factory farms contaminate public water courses on three continents

Water downstream from factory farms harbours an invisible threat to people's health which could eclipse the COVID-19 crisis. The threat? Antibiotic Resistance Genes (ARGs) which are driving antimicrobial resistance the world's superbug crisis - projected to kill up to 10 million people annually by 2050. This publication reports the presence of ARGs in animal waste discharged from industrial farms into public waterways or onto soil (or crops) in four countries. Gauge community impact and sentiment regarding the issue was also highlighted. The water and sediment from public water courses connected to effluent discharges from 6-10 pig farms were tested in each of four countries (Canada, Spain, Thailand and the USA).

Thermal stability of diagnostic targets plasmodium falciparum histidine rich protein II and plasmodium lactate dehydrogenase in rapid detection.

During the COVID-19 epidemic, blood samples are usually processed at 56 to attenuate the virus before pathogen detection. 71 blood samples of malaria patients reported by Shanghai Center for Disease Control and Prevention in 2017-2019 were collected, including 38 with Plasmodium falciparum infection, 8 P. malariae, 11 P. ovale and 14 P. vivax. The effect of inactivation on the thermal stability of P. falciparum histidine rich protein II (PfHRPII) and Plasmodium lactate dehydrogenase (pLDH) in blood samples was assessed before and after incubation at 56 for 30 min using the rapid diagnostic test (RDT) kit. The results showed that among the 38 P. falciparum T1-positive (PfHRPII) blood samples before heat treatment, 35 samples remained to be T1-positive (92.11%, 35/38, chi2=3.123, P>0.05) after heat treatment;while 54 blood samples (26 P. falciparum, 6 P. vivax, 10 P. ovale and 12 P. vivax) that were T2-positive (pLDH) before heat treatment turned to be T2-negative (positive rate 0, 0/54, chi2=87.755, P<0.01) after heat treatment. It was demonstrated that PfHRPII is stable during incubation at 56 for 30 min, while pLDH is unstable and degraded or inactivated during the heating. Therefore, the detection results of P. falciparum will not be affected by RDT, but diagnosis of the parasites other than P. falciparum in blood samples may be missed.Copyright © 2021, National Institute of Parasitic Diseases. All rights reserved.

SARS-CoV-2 infection disrupts trophoblast function and placental syncytial barrier integrity via its accessory protein, ORF3a

Problem: Although it is rare for a SARS-CoV-2 infection to transmit vertically to the fetus during pregnancy, there is a significantly increased risk of adverse pregnancy outcomes due to maternalCOVID- 19. However, there is a poor understanding of such risks because mechanistic studies on how SARS-CoV-2 infection disrupts placental homeostasis are significantly lacking. The SARS-CoV-2 proteome includes multiple structural and non-structural proteins, including the non-structural accessory proteinORF3a. The roles of these proteins in mediating placental infection remain undefined. We and others have shown that autophagy activity in placental syncytium is essential for barrier function and integrity. Here, we have used clinical samples and cultured trophoblast cells to evaluate syncytial integrity of placenta exposed to SARS-CoV-2. The objective of our study was to investigate potential mechanisms through which SARS-CoV-2 impairs placental homeostasis and causes adverse pregnancy outcomes. We tested the central hypothesis that an essential SARS-CoV-2 non-structural and accessory protein, ORF3a, uniquely (amongst multiple viral proteins tested) with a novel three-dimensional structure andwith no homology to any other proteins is a key modulator of placental trophoblast cell dynamics via autophagy and intracellular trafficking of a tight junction protein (TJP), ZO-1. Method(s): We used clinical samples and cultured trophoblast cells to evaluate syncytial integrity of placentas exposed to SARS-CoV- 2. Autophagic flux was measured in placental villous biopsies from SARS-CoV-2-exposed and unexposed pregnant women by quantifying the expression of autophagy markers, LC3 and P62. Trophoblast cells (JEG-3, Forskolin-treated JEG-3, HTR8/SVneo, or primary human trophoblasts (PHTs)) were transfected with expression plasmids encoding SARS-CoV-2 proteins including ORF3a. Using western blotting, multi-label immunofluorescence, and confocal imaging, we analyzed the effect of ORF3a on the autophagy, differentiation, invasion, and intracellular trafficking of ZO-1 in trophoblasts. Using coimmunoprecipitation assays, we tested ORF3a interactions with host proteins. t-tests and one-way analyses of variance (ANOVAs) with post hoc tests were used to assess the data, with significance set at P < .05. Result(s): We discovered :1) increased activation of autophagy, but incomplete processing of autophagosome-lysosomal degradation;2) accumulation of protein aggregates in placentas exposed to SARS-CoV- 2. Mechanistically, we showed that the SARS-CoV-2 ORF3a protein, uniquely 3) blocks the autophagy-lysosomal degradation process;4) inhibits maturation of cytotrophoblasts into syncytiotrophoblasts (STBs);5) reduces production ofHCG-beta, a key pregnancy hormone that is also essential for STB maturation;and 6) inhibits trophoblast invasive capacity. Furthermore, ORF3a harbors an intrinsically disordered C-terminus withPDZ-bindingmotifs.We show for the first time that, 7) ORF3a binds to and co-localizes with the PDZ domain of ZO-1, a junctional protein that is essential for STB maturation and the integrity of the placental barrier. Conclusion(s): Our work outlines a new molecular and cellular mechanism involving the SARS-CoV-2 accessory protein ORF3a that may drive the virus's ability to infect the placenta and damage placental syncytial integrity. This implies that the mechanisms facilitating viral maturation, such as the interaction of ORF3a with host factors, can be investigated for additional functionality and even targeted for developing new intervention strategies for treatment or prevention of SARS-CoV-2 infection at the maternal-fetal interface.

Gaps between Attitudes and Behavior in the Use of Disposable Plastic Tableware (DPT) and Factors Influencing Sustainable DPT Consumption: A Study of Hong Kong Undergraduates

Total waste from human activities, including waste plastics, is huge in Hong Kong. In particular, as a result of the prevention and control measures implemented during the COVID-19 pandemic, take-away meals increased tremendously in Hong Kong, generating disposable plastic tableware (DPT). Although Hong Kong has a charging scheme for plastic bags, it does not have a scheme for plastic tableware. Therefore, this study aimed to understand the attitudes and behavior of people in Hong Kong toward DPT. Our study focused on undergraduate students in Hong Kong, given that they will play a significant role in the future of environmental sustainability. The attitudes and behavior of Hong Kong undergraduate students toward DPT were examined through an online survey with 385 respondents. A multiple stepwise regression was conducted to investigate whether cognitive attitude formation factors could explain the sustainable attitudes formed by undergraduate students in Hong Kong. The survey results revealed that most undergraduates considered DPT to be one of the major causes of environmental damage in Hong Kong;however, many of them, particularly those who strongly agreed with this statement, said that the problem of DPT did not affect their quality of life. The regression analysis showed that imposing a DPT charge would be the most significant driver to reduce its use. The research findings identified gaps between attitudes and behavior regarding the use of DPT and the factors influencing sustainable DPT consumption.

Questão ambiental e pandemia: a destrutividade do sistema do capital na ordem do dia

O presente artigo tem como objetivo refletir sobre a relação entre a questão ambiental, crise estrutural do capital e a pandemia do novo Coronavírus. Compreendemos neste trabalho que a pandemia do novo Coronavírus que gera a doença nomeada de Covid-19 está organicamente relacionada com a crise do capital, como fruto do processo de acumulação capitalista avidamente crescente que necessita da exploração inesgotável dos recursos humanos e naturais, tornando-os mercadorias. Essa dupla exploração em ritmo galopante, inerente à ordem sociometabólica do capital, constituem elementos fundamentais para o atual cenário de crise mundial. Para tal reflexão, caminhamos sob a perspectiva do método dialético-crítico de Marx, utilizando-se como metodologia a pesquisa de caráter bibliográfico e documental. Dividimos o trabalho em duas seções centrais: a primeira discute a questão ambiental e a ordem sociometabólica do capital, relação de acumulação e destrutividade;a segunda realiza a relação entre os três eixos de discussão: a crise do capital, a pandemia e a questão ambiental.Alternate :This article aims to reflect on the relationship between the environmental issue, structural crisis of capitalism and the new Coronavirus pandemic. We understand in this work that the pandemic of the new Coronavirus that generates the disease named Covid-19 is organically related to the crisis of capitalism, as a result of the accumulation process greedily growing capitalist that needs the inexhaustible exploitation of human resources and natural, making them commodities. This double exploration at a galloping pace, inherent to sociometabolic order of the capital, constitute fundamental elements for the current scenario of world crisis. For such reflection, we walk under the perspective of the dialectical-critical method of Marx, using bibliographic and documentary research as a methodology. We divided the work into two central sections: the first discusses the environmental issue and the order sociometabolic of capitalism, relation of accumulation and destructiveness;the second analyzes the relationship between the three perspectives of the discussion: the crisis of capitalism, the pandemic and the environmental issue.

What is hidden in the luggage? First assessments of illegal seashells gathering in Sardinia (Italy)

Natural souvenirs collection has been identified as a driving force in biodiversity and habitat degradation of tropical marine ecosystems. This work considers this phenomenon in the Mediterranean region taking Sardinia (Italy), one of the most renowned tourism destinations, as a case study. The biological material seized at Cagliari-Elmas Airport (years 2019–2020: 138 kg) was analyzed: 199 taxa were identified, gastropods (112 species, 7866 pieces) and bivalves (63 species, 34,218 pieces) resulted the most represented classes. Twenty-two protected species were found in the tourists' luggage including Patella ferruginea and Pinna nobilis, the invertebrates most threatened with extinction in the Mediterranean Sea. This study demonstrates that the illegal collection of natural mementos is common in Sardinia, thus its relevance is not limited to tropical regions. Regulation, enforcement and compliance shortcomings emerged, highlighting the importance of strengthening stakeholders' collaboration for a deeper insight on this phenomenon and implementing effective conservation strategies.

Efficient degradation of benzalkonium chloride by FeMn-CA300 catalyst activated persulfate process: Surface hydroxyl potentiation mechanism and degradation pathway.

The consumption of disinfectants increased dramatically with the outbreak of the COVID-19 epidemic. Benzalkonium chloride (DDBAC), a cationic surfactant disinfectant for import and export cargoes, is used for effective degradation method. For DDBAC effective degradation, polyhedral Fe-Mn bimetallic catalyst of Prussian blue analogue (FeMn-CA300) was novelty developed for rapid peroxymonosulfate (PMS) activation. Results showed that the Fe/Mn redox and surface hydroxyl groups in the catalyst played an important role in the DDBAC-enhanced degradation. The removal effectiveness of 10 mg L-1 DDBAC was up to 99.4% in 80 min under the initial pH = 7, catalyst dosage of 0.4 g L-1, and PMS concentration of 15 mmol L-1. In addition, FeMn-CA300 had a wide pH applicability range. The results indicated that hydroxyls, sulfate radicals, and singlet oxygen could effectively improve the degradation efficiency, where sulfate radicals played a crucial role. Finally, the corresponding degradation path of DDBAC was further provided according to GC-MS results. The results of this study provide new insights into the degradation of DDBAC, thereby highlighting the great potential of FeMnca300/PMS to control refractory organic compounds in the aqueous phase.

[Pulmonary CT angiography in the first wave of the COVID-19 pandemic: comparison between patients with and without infection and with a pre-pandemic series]. / Angio-TC pulmonar durante la pandemia de COVID-19: comparación en pacientes con y sin la infección y con una serie prepandémica.

BACKGROUND AND AIMS: To analyze the diagnostic performance of pulmonary CT angiography and to compare different D-dimer cutoffs for the diagnosis of acute pulmonary embolism in patients with and without SARS-CoV-2 infections. MATERIALS AND METHODS: We retrospectively analyzed all consecutive pulmonary CT angiography studies done for suspected pulmonary embolism in a tertiary hospital during two time periods: the first December 2020 through February 2021 and the second December 2017 through February 2018.D-dimer levels were obtained less than 24 hours before the pulmonary CT angiography studies. We analyzed the sensitivity, specificity, positive and negative predictive values, area under the receiver operating curve (AUC), and pattern of pulmonary embolism for six different values of D-dimer and the extent of the embolism. During the pandemic period, we also analyzed whether the patients had COVID-19. RESULTS: After excluding 29 poor-quality studies, 492 studies were analyzed; 352 of these were done during the pandemic, 180 in patients with COVID-19 and 172 in patients without COVID-19.The absolute frequency of pulmonary embolism diagnosed was higher during the pandemic period (34 cases during the prior period and 85 during the pandemic; 47 of these patients had COVID-19). No significant differences were found in comparing the AUCs for the D-dimer values. The optimum values calculated for the receiver operating characteristic curves differed between patients with COVID-19 (2200 mcg/L), without COVID-19 (4800 mcg/L), and diagnosed in the prepandemic period (3200 mcg/L).Peripheral distribution of the emboli was more common in patients with COVID-19 (72%) than in those without COVID-19 and than in those diagnosed before the pandemic [OR 6.6, 95% CI:1.5‒24.6, p<0.05 when compared to central distribution]. CONCLUSIONS: The number of CT angiography studies and the number of pulmonary embolisms diagnosed during the pandemic increased due to SARS-CoV-2 infection. The optimal D-dimer cutoffs and the distribution of the pulmonary embolisms differed between the groups of patients with and without COVID-19.

Impact of disposable mask microplastics pollution on the aquatic environment and microalgae growth.

The COVID-19 pandemic has mandated people to use medical masks to protect the public. However the improper management of disposable mask waste has led to the increase of marine pollution, in terms of water quality, and the decline in aquatic microorganisms. The aim of this research was to investigate the impact of disposable mask waste on fresh water and microalgae biomass quality. Disposable masks (untreated or treated with Enterococcus faecalis) were placed in 10-L glass reactors containing fresh water or water containing algal Chlorella sp. and its growth supplements (Chlorella medium) (four 10-L reactors in total) and kept in controlled conditions for 3 months. Water and biomass yield quality were evaluated using water quality analysis, spectroscopy, scanning electron microscopy (SEM), and proximate lipid and protein analysis. Disposable masks, incubated in either fresh water or Chlorella medium, affected several water quality parameters such as chemical oxygen demand (COD), biological oxygen demand (BOD), dissolved oxygen (DO), and pH. Microplastic identification revealed that some fibers were present in the water following a 100-day treatment process. Fourier transform-infrared spectroscopy (FTIR) analysis was used to determine the change in important, organic functional groups and highlighted the disappearance of a peak at 1530 cm-1 corresponding to the primary protein (C-N) and the appearance of new peaks at 1651 cm-1 and 1270 cm-1 corresponding to methyl alcohol (CH2OH) and ketone (C = O), respectively. This indicated the detrimental effect of disposable mask fragmentation on the biomass quality. The SEM investigation has shown a damage to the surface membrane of Chlorella sp. cells. Altogether, disposable masks decreased the water quality and damaged microalgae by inhibiting their growth. Therefore, the disposable mask contaminated by various microbes, after being used by a human, may be one of the most dangerous hazards to the environment.

Disaster Risk Reduction for Resilience: Disaster Risk Management Strategies

This book is part of a six-volume series on Disaster Risk Reduction and Resilience. The series aims to fill in gaps in theory and practice in the Sendai Framework, and provides additional resources, methodologies and communication strategies to enhance the plan for action and targets proposed by the Sendai Framework. The series will appeal to a broad range of researchers, academics, students, policy makers and practitioners in engineering, environmental science and geography, geoscience, emergency management, finance, community adaptation, atmospheric science and information technology.This volume offers the international guidelines and global standards for resilient disaster risk reduction and lessons learned from disasters, particularly the COVID-19 and Cholera pandemics. A resilient health system and an effective disaster risk management Index are then suggested. The book further emphasizes urban resilience strategies with local authorities, adaptation strategies for urban heat at regional, city and local scales, and lessons from community-level interventions. Also addressed are coastal erosion, displacement and resettlement strategies. Land use planning and green infrastructure are suggested as tools for natural hazards reduction. Human security in times of climate change and urban heat at regional, city and local scales is discussed for an integrated action, with case studies based in Manila, Burkina Faso, Chad, Mauritania, Niger, Senegal, Nigeria, India, Spain, and Ghana. Structure design for cascading disasters resulting from mining and flooding is presented and sustainable smart city planning using spatial data is recommended. © Springer Nature Switzerland AG 2022.

Design of a Laboratory Developed Assay to Aid in the Detection of Sars-Cov-2 Virus Rna in Oropharyngeal and Nasopharyngeal Swabs Adapted from the Cdc Protocol

Intro: SARS-CoV-2 is a single-strand enveloped RNA virus belonging to the family Coronaviridae. It was first recognized in late 2019 as causing COVID-19, and later declared a pandemic. The development of this assay aided in the detection of positive cases early in the pandemic which in turn facilitated the isolation of infected individuals to minimize the spread. Method(s): The SARS-CoV-2 RNA detection by real time RT-PCR is a molecular in vitro diagnostic test that aids in the detection and diagnosis of SARS-CoV-2 in nasopharyngeal and oropharyngeal specimens. This test is based on nucleic acid extraction and amplification technology and uses oligonucleotide primers and dual-labeled hydrolysis probes. RNA is isolated and purified from specimens using the Abbott m2000sp. This technology uses magnetic particles to capture and purify the RNA. The bound RNA is eluted and transferred to a 96 deep-well plate and is ready for amplification. The master mix is prepared manually and is added to a PCR plate together with the extracted RNA. The RNA is reverse transcribed to cDNA and subsequently amplified in the Abbott m2000rt. In this process, the probe anneals to a specific target sequence located between the forward and reverse primers. During the extension step of the PCR cycle, the 5' nuclease activity of Taq polymerase degrades the probe, causing the reporter dye molecules to be cleaved from their respective probes, increasing the fluorescence intensity. Fluorescence intensity is monitored at each PCR cycle on the Abbott m2000rt instrument. Finding(s): The clinical evaluation was performed by testing patient samples in a blinded fashion. The performance of SARS-CoV-2 Assay was established using 60 clinical specimens. The positive and negative percent agreements were analyzed by comparing the SARS-CoV-2 Assay results to Seegene's AllplexTM 2019-nCoV which showed 100% concordance. Conclusion(s): This assay demonstrated accuracy and reproducibility for the detection of SARS-CoV-2.Copyright © 2023

Plastic or plastic-free life: From formation to removal.

Microplastics (MPs) and nanoplastics (NPs) have caused global environmental concerns due to their ubiquitous existence in our surrounding environment and the potential threats posed to the ecosystem and human health. This review aims to extend current knowledge on the formation and degradation of MPs and NPs. The paper presents the potential sources of MPs and NPs including plastic containers, textiles, cosmetics, personal care products, COVID-19 wastes, and other plastic products. Once in the natural environment, the fragmentation and degradation of plastic wastes are thought to be initiated by physical, chemical, and biological factors. The corresponding degradation mechanism will be presented in the present review. Given the plastic life and environment, humans are inevitably exposed to MPs and NPs through ingestion, inhalation, and dermal contact. The potential risks MPs/NPs pose to humans will be also discussed in our study. Currently, the relevance of MP/NP exposure to human health outcomes is still controversial and not yet fully understood. Deciphering the translocation and degradation of plastics in the human body will be helpful to reveal their potential organotoxicity. In this case, available approaches to alleviate MP/NP pollution and advanced strategies to reduce MP/NP toxicity in humans are recommended to build a plastic-free life.

Dose-response and type-dependent effects of antiviral drugs in anaerobic digestion of waste-activated sludge for biogas production.

In the context of the COVID-19 pandemic, antiviral drugs (AVDs) were heavily excreted into wastewater and subsequently enriched in sewage sludge due to their widespread use. The potential ecological risks of AVDs have attracted increasing attention, but information on the effects of AVDs on sludge anaerobic digestion (AD) is limited. In this study, two typical AVDs (lamivudine and ritonavir) were selected to investigate the responses of AD to AVDs by biochemical methane potential tests. The results indicated that the effects of AVDs on methane production from sludge AD were dose- and type-dependent. The increased ritonavir concentration (0.05-50 mg/kg TS) contributed to an 11.27-49.43% increase in methane production compared with the control. However, methane production was significantly decreased at high lamivudine doses (50 mg/kg TS). Correspondingly, bacteria related to acidification were affected when exposed to lamivudine and ritonavir. Acetoclastic and hydrotropic methanogens were inhibited at a high lamivudine dose, while ritonavir enriched methylotrophic and hydrotropic methanogens. Based on the analysis of intermediate metabolites, the inhibition of lamivudine and the promotion of ritonavir on acidification and methanation were confirmed. In addition, the existence of AVDs could affect sludge properties. Sludge solubilization was inhibited when exposed to lamivudine and enhanced by ritonavir, perhaps caused by their different structures and physicochemical properties. Moreover, lamivudine and ritonavir could be partially degraded by AD, but 50.2-68.8% of AVDs remained in digested sludge, implying environmental risks.

Preparation of PLA-based SMS nonwoven composites and its applications in protective apparel

The global COVID-19 pandemic has triggered a huge demand for the protective nonwovens. However, the main raw material of nonwovens comes from petroleum, and the massive consumption of petroleum-based polymers brings great pressure to ecosystem. Therefore, it is significant to develop biodegradable protective barrier products. In this work, a polylactic-based composite (a tri-layer nonwovens composed of spunbond, meltblown and spunbond, SMS) was prepared and applied for protective apparel. The surface morphology and chemical changes of the fibers were characterized and analyzed by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and energy dispersive spectroscopy (EDS). The liquid contact angle and permeability, breathability and moisture permeability, frictional charge and mechanical strength of the samples were evaluated and compared. The samples degradability was also recorded. The results demonstrate that the optimum formula for anti-fouling treatment on SMS is F-30. The treated fabric possesses superior liquid repellency and anti-permeability, with contact angles of water and alcohol at 128° and 115° respectively, while the alcohol repellent grade reaches level 7. The treated sample has less strength loss but exhibits favorable breathability, moisture permeability and anti-static properties, which can meet the requirements of protective apparels. After fluorine resin coating, the composite still provide excellent degradation performance, and the weight loss rate reaches more than 80% after 10 days water degradation. These results provide new insights for the application of PLA-based SMS in biodegradable protective apparel. © 2023 The Textile Institute.

Ecology in the age of climate change: Challenges facing the effective implementation of nature-based solutions in terrestrial ecosystems

At this stage of the Great Acceleration of the Anthropocene, humanity is experiencing the global issues of worsening climate change impacts, devastating damage from more frequent and severe natural disasters and the COVID-19 pandemic, all of which are attributable to ecosystem degradation and biodiversity loss. The global community recognises that these issues pose severe societal and economic risks. “Nature-based solutions” have been posited as a means to address these threats. Nature-based solutions utilise natural terrestrial ecosystem functions to provide environmental, social and economic benefits at low cost. The growing social demand for nature-based solutions constitutes an opportunity for the field of ecology to expand beyond the conventional focus on biodiversity and conservation and shift to presenting biodiversity and ecosystem functions as the basis of human well-being and social sustainability. We sought to identify a trajectory for ecological research that is aimed at contributing to the effective implementation of nature-based solutions. First, we summarise current social needs related to terrestrial ecosystem utilisation. Next, we provide an overview of existing literature and knowledge regarding biodiversity and terrestrial ecosystem function, which are critical to nature-based solutions. Finally, we identify outstanding ecological hurdles to the implementation of these strategies and propose a way forward based on our findings. We explain that any basic presentation of ecological processes requires addressing the impacts of climate change and the interrelatedness of biodiversity, climate and social systems. Enhanced ecological process models are critical for linking biodiversity and ecosystems with climate and social systems. It is crucial to establish a framework that embeds monitoring systems, data infrastructure and delivery systems within society to mobilise terrestrial ecosystem and biodiversity data and results. Furthermore, the implementation of nature-based solutions must include acknowledging trade-offs in objectives and transdisciplinary research with other fields and stakeholders with the shared goal of transformative change. Ecological research must demonstrate more clearly how terrestrial biodiversity and ecosystems are linked to human health and well-being, as well as how they are affected by production and consumption systems. In the age of climate change, the knowledge and tools of the ecologist form the foundation of nature-based solutions and provide an indispensable theoretical basis for this approach.Alternate :æŠ„éŒ²äººæ–°ä¸–ã®å¤§åŠ é€Ÿã¨ã‚‚å‘¼ã°ã‚Œã‚‹æ°—å€™å¤‰å‹•ã®æ™‚ä»£ã«ãŠã„ã¦ã€æ°—å€™å¤‰å‹•å½±éŸ¿ã®é¡•åœ¨åŒ–ã€è‡ªç„¶ç½å®³ã®æ¿€ç”šåŒ–ãƒ»é »ç™ºåŒ–ã€COVID-19の世界的流行などの地球規模の問題が増大している。国際社会では、ã"ã‚Œã‚‰ã®å•é¡Œã¯ç”Ÿæ…‹ç³»ã®åŠ£åŒ–ã‚„ç”Ÿç‰©å¤šæ§˜æ€§ã®æå¤±ãŒè¦å› ã§ã‚ã‚‹ã"と、そして社会経済にも多大な損害ã‚'与える大きなリスクであるã"とが共通の認識となりつつある。そのような状況ã‚'åæ˜ ã—ã€é™¸åŸŸç”Ÿæ…‹ç³»ã®å¤šé¢çš„ãªæ©Ÿèƒ½ã‚'活用するã"とで、低いコストでç'°å¢ƒãƒ»ç¤¾ä¼šãƒ»çµŒæ¸ˆã«ä¾¿ç›Šã‚'もたらし、社会が抱える複数の課題の解決に貢献する「自然ã‚'基盤とした解決策」という新しい概念に大きな期待が寄せられている。ã"の解決策への社会的なニーズの高まりは、生態学が長年取り組ã‚"できた生物多様性や生態系の保全に関する課題ã‚'超えて、生態学が生物多様性や生態系が豊かな人é–"社会ã‚'継続し発展させる知的基盤となるã"とや、生態学の社会的有用性ã‚'示す機会である。そã"で本稿では、気候変動時代における「自然ã‚'åŸºç›¤ã¨ã—ãŸè§£æ±ºç­–ã€ã®å®Ÿè·µã«å‘ã‘ãŸç”Ÿæ…‹å­¦ç ”ç©¶ã®æ–¹å‘ã¥ã‘ã‚'目的とし、陸域生態系の活用に対する社会的なニーズの現状ã‚'概観する。その上で、「自然ã‚'åŸºç›¤ã¨ã—ãŸè§£æ±ºç­–ã€ã®éµã¨ãªã‚‹é™¸åŸŸç”Ÿæ ‹ç³»ã®ç”Ÿç‰©å¤šæ§˜æ€§ã‚„ç”Ÿæ…‹ç³»æ©Ÿèƒ½ã«é–¢ã™ã‚‹çŸ¥è¦‹ã‚'整理して課題ã‚'抽出し、ã"れらã‚'è¸ã¾ãˆã¦ä»Šå¾Œã®ç”Ÿæ…‹å­¦ç ”ç©¶ã®æ–¹å‘æ€§ã‚'å…·ä½"的に示す。まず、現象の基礎的な理解という観点からは、生物多様性ã‚'含む陸域生態系と気候システムや社会システムとの相äº'関係性ã‚'含めた包括的な気候変動影響のメカニズムの解明と、予測・評価のためのプロセスモデルの高度化ã‚'進めるã"と、そして同時に、陸域生態系と生物多様性の変化ã‚'ç¤ºã™ãŸã‚ã®åŠ¹æžœçš„ãªãƒ¢ãƒ‹ã‚¿ãƒªãƒ³ã‚°ã¨æƒ…å ±åŸºç›¤ã®å¼·åŒ–ã‚'行い、データや分析結果ã‚'社会に還元するフレームワークã‚'構築するã"ã¨ãŒå„ªå…ˆäº‹é …ã§ã‚ã‚‹ã€‚ã‚ˆã‚Šå®Ÿè·µçš„ãªè¦³ç‚¹ã‹ã‚‰ã¯ã€ã€Œè‡ªç„¶ã‚'基盤とした解決策」の実装や社会変革などにおいて共通の目標ã‚'ã‚‚ã¤ä»–åˆ†é‡Žã¨ã®å­¦éš›ç ”ç©¶ã‚'積極的に行うã"とにより、実装における目的é–"のトレードオフã‚'示すã"と、健康・福祉の課題や生産・消費システムの中での陸域生態系や生物多様性への影響や役割ã‚'示すã"ã¨ãªã©ãŒå„ªå…ˆäº‹é …ã¨ãªã‚‹ã€‚æ°—å€™å¤‰å‹•ã«ä»£è¡¨ã•ã‚Œã‚‹ä¸ç¢ºå®Ÿæ€§ã®é«˜ã„ç'°å¢ƒä¸‹ã§ã€åŠ¹æžœçš„な「自然ã‚'åŸºç›¤ã¨ã—ãŸè§£æ±ºç­–ã€ã®å®Ÿæ–½ãŸã‚ã«ã¯ã€ãã®ç§‘å­¦çš„åŸºç›¤ã¨ãªã‚‹ç”Ÿæ…‹å­¦ã®çŸ¥è¦‹ã¨ãƒ„ãƒ¼ãƒ«ã¯ä¸å¯æ¬ ã§ã‚ã‚Šã€ã¾ãŸãã®å®Ÿè£…ã‚'通じた社会変革へのé"筋においても生態学の貢献が期待されている。

A comparative study of waste management practices in pre-COVID and during-COVID scenarios: an overview of the hotel industry

The COVID-19 pandemic has been challenging for the hotel industry, with rooms being converted into quarantine centres and leading to an increase in waste volume and composition. With frequent lockdowns and stringent guidelines by governments on social distancing, 60% of hotels converted 10% of their rooms into quarantine facilities, which drastically changed the composition of hotel waste (JLL, 2020). This waste may lead to environmental degradation if handled inappropriately. The primary objective of this study is to identify, compare and highlight the challenges faced in waste management practices in two different circumstances, namely pre-COVID and during-COVID, in the hotel industry. Data were collected using a structured questionnaire put to hoteliers in selected hotels in India. A total of 61 responses were recorded out of 100 respondents. Descriptive analysis indicated new practices in hotel housekeeping such as use of single-use PPE kits, arrangement of separate zones for disposing of medical waste and digitalization of services. Another interesting finding of the research is the aggravation of single-use plastic in the form of disposable crockery, cutlery and packaging of PPE kits. Lastly, the study showed an increase in operational costs and highlighted innovative procedures in existing waste management disposal practices and suggested new practices that will be of great significance for dealing with similar episodes in the future.

THE SPIKE OF SARS-CoV-2 VARIANTS ALLOWS FOR MORE EFFICIENT COUNTERACTION OF BST2

Background: BST2/Tetherin is an interferon-stimulated gene with antiviral activity against enveloped viruses. Particularly, BST2 tethers virions at their site of assembly, preventing their release and spread. In addition to this primary role, BST2 is as an important bridge between the innate and adaptive immune system, since (i) BST2 routes tethered particles to lysosomes, which generates viral breakdown products that engage pattern recognition receptors;and (ii) trapped virions facilitate antibody-dependent cell-mediated cytotoxicity (ADCC). In turn, viruses have evolved mechanisms to bypass BST2, either by targeting BST2 for proteasomal/lysosomal degradation or by removing BST2 from sites of virion assembly. However, the role of BST2 in SARS-CoV-2 replication, spread, evolution, and pathogenesis remains largely unknown. Method(s): The antiviral potential of BST2 against SARS-CoV-2 was investigated by infecting different SARS-CoV-2 isolates (Hong Kong, Alpha, Beta, Delta, and Omicron) in BST2+ and BST2- cells. Culture supernatants were collected to assess virion production by ELISA and infectivity by TCID50. Infected cells were analyzed by western blot and flow cytometry to examine viral and cellular protein levels, including BST2. Transfection of individual SARS-CoV-2 ORFs and mutagenesis studies allowed us to identify the genes that the virus uses to downregulate BST2. Immunoprecipitation assays revealed protein-protein interactions and changes in ubiquitination patterns. Experiments with proteasomal and lysosomal inhibitors furthered our mechanistic understanding of how SARS-CoV-2 counteracts BST2. Finally, fluorescence microscopy studies uncovered changes in the subcellular distribution of BST2 in SARS-CoV-2 infected cells. Result(s): While BST2 reduces virion release, SARS-CoV-2 has evolved to counteract this effect. Specifically, SARS-CoV-2 uses the Spike to interact with BST2, sequester the protein at perinuclear locations, and ultimately route it for lysosomal degradation. By surveying different SARS-CoV-2 variants of concern (Alpha-Omicron), we found that each variant is more efficient than the previously circulating strain at downregulating BST2 and facilitating virion production, and that mutations in the Spike account for their enhanced BST2 antagonism. Conclusion(s): As part of its adaptation to humans, SARS-CoV-2 is improving its capacity to counteract BST2, highlighting that BST2 antagonism is important for SARS-CoV-2 infectivity and transmission.

Air pollution with pm10 for the municipality of Burgas and the monthly cases of disease with COVID 19, 2021

An important environmental problem for the Municipality of Burgas is the relatively high levels of PM10 pollution. Particulate matter PM10 is defined as the fraction of particles with an aerodynamic diameter smaller than 10 pm. The article provides statistical processing and evaluation of daily data on the concentration of PM10 in the air by quarters fix Burgas, 2021. A histogram of the frequency distribution of concentrations by quarters was prepared. A regression model for calculating the monthly concentrations in the atmospheric air is derived The tests and inspections performed show that the performed modelling is suitable for evaluation, analysis and forecast. Air pollution harms human health and the environment. Exposure ID air pollution is associated with a wide range of acute and chronic health effects, ranging from irritating effects to death From the end of 2019 until now in the world, Europe and in particular Bulgaria is raging a dangerous respiratory disease known as COVD19. The average monthly new cases of COVD19 for Burgas were assessed, as well as the respective maximum and minimum monthly values. A qualitative assessment of the relationship between the monthly concentrations of PM10 and the incidence of COVID19 was made.

Viral Dynamics and Factors Favouring the Duration of Covid-19 Positivity in Cameroon

Background: Evolution evidence of Coronavirus disease 2019 (COVID-19) and viral clearance time remains limited in tropical settings. Understanding this is crucial for public health control measures at community-level. We evaluated the viral dynamics of SARS-CoV-2 infection and factors associated with positivity duration in COVID-19 cases in Cameroon. Method(s): We conducted a prospective cohort-study of SARS-CoV-2 positive cases from the first to third wave (March 2020-October 2021) in Yaounde- Cameroon. RT-PCR was performed on nasopharyngeal swabs. SARS-CoV-2 positivity duration was evaluated from the first to last positive test before a negative result. Epi-info V.7.0 was used for data analyses with p< 0.05 considered statistically significant Results: A total of 282 participants were enrolled. The mean age was 41+/-14 years, with male predominant (62.1%). We had 15.6% symptomatic cases and cough most common (59.09%). The overall median positivity duration was 15[IQR: 9-23] days with 15[ IQR: 13-16] in the first, 17[ IQR: 11-26] in the second and 8[ IQR: 4-12] in the third wave (p= 0.007). Positivity duration was significantly higher in males (16 versus 14 days, p=0.03) and people aged >40 years (15 versus 14 days, p=0.02). Positivity duration was not affected by presence or absence of symptoms (p=0.80). No significant correlation was found with viral load (r=0.03;p=0.61). Considering baseline (24.7+/-7.2Ct) and last viral load (29.3+/-5.9 Ct), the DELTACt (4.6+/-1.3) and positivity duration (15 days) revealed a kinetic in viral decay of 0.3+/-0.087 Ct/day. Conclusion(s): A median positivity duration of 15 days is in accordance with viral clearance around 2 weeks for optimal confinement at community-level. Men and/or the elderly stand at higher risk of prolonged infection. Given the viral decay (0.3 Ct daily), we suggest personalized confinement periods. The variability of positivity duration according to phases could be function of strains which could be a factor of positivity duration.